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Non-spermicidal lubricants are used, and spermicides such as water and light are not allowed to come in contact with the ejaculate. To prepare the artificial vagina, fill it with water to obtain proper temperature and pressure. Therefore, it is important to prepare the AV to the personal preference for the stallion being collected. Freshly collected extended semen can be cooled to Several artificial vaginas are available: the Colorado, Missouri and Japanese models. Collection with a jump mare. Do not use products detrimental to sperm cells.

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The phantom should be designed to be comfortable for the stallion and should mimic the same angle as the mare. Therefore, for maximum amounts of sperm collection and minimum labor, stallions should be collected every other day. Sperm output per week is similar whether collecting every other day or daily.

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